Protocols

• Direct-zol RNA MiniPrep (we start with 1 mL bacteria culture and proceed with the instructions for “Cell Suspensions”)

• RNA Prep (Hot Phenol Phase Lock Gel)

• NorthernMax-Gly Liu Lab

• Northern Blot Protocol (sRNA)

• Ethanol Precipitation of Nucleic Acids

• Total RNA preparation (from DeRisi Lab, UCSF)

• Protocol sRNA Cloning (for 454)

Other Protocols